Video showing separation between the nucleocapsid (tracked via the labelled N-EGFP protein, left panel), and the VLP membrane tracked via the M-mCherry labelled protein (middle panel). Merged of both channels is the right panel.

A549

A549 cells are lung carcinoma epithelial cells.

No cell labelling,

SARS-CoV-2 VLPs Wuhan

Wild Type SARS-CoV-2 virus-like particles containing all the structural proteins.

M-pHluorin N-EGFP, Part of the M protein of the particle is tagged with mCherry and part of N protein is tagged with EGFP. This allows us to track the membrane of the particle simultaneously with its nucleocapsid under the microscope.

No treatment